The Gadsden flag, that includes a rattlesnake and the phrase "Don't Tread on Me

https://kylerstiu890.oakridgedigest.com/posts/from-battlefields-to-museums-the-journey-of-historic-civil-war-flags

The Gadsden flag, that includes a rattlesnake and the phrase "Don't Tread on Me," used to be followed by a few Confederate units as a symbol of resistance against perceived oppression.